dc.contributor.advisor | Tovar Ticse, Rosmery Dionicia | es_PE |
dc.contributor.author | Cunias Reyes, William Luis | es_PE |
dc.contributor.author | Serva Rosales, Mercedes Gladys | es_PE |
dc.date.accessioned | 2024-06-17T20:54:06Z | |
dc.date.available | 2024-06-17T20:54:06Z | |
dc.date.issued | 2024-06-04 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12970/2174 | |
dc.description.abstract | Objetivo: determinar la actividad antibacteriana in vitro del extracto etanólico de cáscara de Allium sativum L. (ajo) frente cepas de Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922.
Materiales y métodos: se realizó un estudio cuantitativo y experimental utilizando una población de 20 kg de bulbos de Allium sativum L. (ajo), de los cuales se extrajo una muestra de 2 kg de cáscara de Allium sativum L. (ajo). Paralelamente, se prepararon 10 placas Petri inoculadas con S. aureus y E. coli, respectivamente. En el estudio se empleó la técnica de marcha fitoquímica, difusión en agar en pozos, con conjuntos al 25%, 50%, 75% y 90% de extracto de ajo, comparados con Ciprofloxacino 5ug como control.
Resultados: se identificó la presencia de compuestos fenólicos, alcaloides, lactonas α, β-insaturadas, taninos, azúcares reductores y flavonoides. Los promedios en todas las concentraciones del extracto etanólico de cáscara de Allium sativum L. (ajo), fueron menores que ciprofloxacino 5ug (35,7260 y 29,9640 mm), asimismo mediante el análisis de ANOVA se identificaron diferencias significativas (p<0,05).
Conclusión: el extracto etanólico de cáscara de Allium sativum L. (ajo) presentó actividad antibacteriana in vitro frente Staphylococcus aureus y Escherichia coli. | es_PE |
dc.description.abstract | Objective: to determine the in vitro antibacterial activity of the ethanolic extract of Allium sativum L. (garlic) peel against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 strains.
Materials and methods: a quantitative and experimental study was carried out using a population of 20 kg of Allium sativum L. (garlic) bulbs, from which a sample of 2 kg of Allium sativum L. (garlic) peel was extracted. In parallel, 10 Petri dishes inoculated with S. aureus and E. coli, respectively, were prepared. The study employed the phytochemical march technique, agar diffusion in wells, with sets of 25%, 50%, 75% and 90% garlic extract, compared with Ciprofloxacin 5ug as control.
Results: the presence of phenolic compounds, alkaloids, α-lactones, β-unsaturated lactones, tannins, reducing sugars and flavonoids were identified. The averages in all concentrations of the ethanolic extract of Allium sativum L. (garlic) peel were lower than ciprofloxacin 5ug (35.7260 and 29.9640 mm), and significant differences were identified by ANOVA analysis (p<0.05).
Conclusion: the ethanolic extract of Allium sativum L. (garlic) peel showed in vitro antibacterial activity against Staphylococcus aureus and Escherichia coli. | es_PE |
dc.format | application/pdf | es_PE |
dc.language.iso | spa | es_PE |
dc.publisher | Universidad María Auxiliadora | es_PE |
dc.rights | info:eu-repo/semantics/openAccess | es_PE |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | es_PE |
dc.subject | Actividad antibacteriana | es_PE |
dc.subject | Allium sativum L. | es_PE |
dc.subject | Escherichia coli | es_PE |
dc.subject | Staphylococcus aureus. | es_PE |
dc.subject | Antibacterial activity | es_PE |
dc.title | Actividad antibacteriana in vitro del extracto etanólico de cáscara de Allium sativum L. (ajo) frente cepas de Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922 | es_PE |
dc.title.alternative | In vitro antibacterial activity of ethanolic extract of Allium sativum L. (garlic) peel against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 strains | es_PE |
dc.type | info:eu-repo/semantics/bachelorThesis | es_PE |
dc.publisher.country | PE | es_PE |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.01.05 | es_PE |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es_PE |
thesis.degree.discipline | Farmacia y Bioquímica | es_PE |
thesis.degree.grantor | Universidad María Auxiliadora. Facultad de Ciencias de la Salud. | es_PE |
thesis.degree.name | Químico Farmacéutico | es_PE |
renati.advisor.dni | 76967427 | |
renati.advisor.orcid | https://orcid.org/0000-0001-9520-5372 | es_PE |
renati.author.dni | 41736768 | |
renati.author.dni | 09625747 | |
renati.discipline | 917046 | es_PE |
renati.juror | Rodríguez Lichtenheldt, José Edwin Adalberto | es_PE |
renati.juror | Dionicio Escalante, Elisa Roxana | es_PE |
renati.juror | Siancas Tao, Norio Salvador | es_PE |
renati.level | https://purl.org/pe-repo/renati/level#tituloProfesional | es_PE |
renati.type | https://purl.org/pe-repo/renati/type#tesis | es_PE |